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Laboratories studying cartilage matrix biology, chondrocyte proliferation and differentiation, and growth factor signalling in musculoskeletal research models have incorporated this fragment as an experimental variable representing an expanding research frontier beyond its primary metabolic applications

It directly stimulates anorexigenic POMC neurons and inhibits adjacent orexigenic NPY neurons in the ARC of the hypothalamus, thus promoting satiety, increasing energy expenditure, and resulting in weight loss (6)

After an initial loading phase of six injections over two weeks (or more frequently if neurological symptoms are present), maintenance injections are given every two to three months for life to prevent deficiency recurrence