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doi:10.1016/j.cellsig.2016.05.021

For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

Stroke is one of the most important causes of morbidity and mortality in the world, and aging is one of the risk factors that significantly increase its incidence