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Total RNA was extracted using the TRIzol reagent (Invitrogen, USA), quantified on an Agilent 2100 bioanalyser (Agilent Technologies, CA, USA) and a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc.) and separated on 1% agarose gel

In addition, efflux transporters, carried by BBB cells, regulate molecular and cellular transport across the BBB and protect the brain from toxins, xenobiotics, and pathogens (243)