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Our initial hypothesis was that MDD is linked to a reduction in the secretion and production of critical cytokines that influence the immune system response, including IL-1, IL-6, IL-2, and interferon-gamma (IFN-)

Saetre K, Godhwani N, Maria M, Patel D, Wang G, Li KI, Wormser GP, Nolan SM

Fifty microliters of supernatant was mixed with 175 l of HPLC-grade water and 25 l of 20% sulfosalicylic acid fresh solution, followed by incubation for 15 min at room temperature