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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18

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ASNS metabolizes glutamine and, in combination with the GAD and GABA shunts, provides the cell with a backfill entry point in the TCA cycle that produces oxaloacetate and ATP in the absence of glutamine catabolism

Cells seeded in six-well plate were incubated with C11-BODIPY 581/591 at a final concentration of 10 M for 30 min at 37 C and washed three times with PBS