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Early after cuprizone initiation and in the context of minimal inflammation, changes in myelin composition and/or lipid polarity have been detected well in advance of oligodendrocyte cell body damage giving weight to the idea that cuprizone may have direct, albeit subtle, effects on myelin [79, 98, 99] the human relevance of such altered myelin biochemistry prior to overt demyelination has since been confirmed [98]

Informed consent for use in research was obtained from donor families by the specialist nurse in organ donation in accordance with NHSBT guidelines, and this study was approved by the London-Surrey Borders National Research Ethics Service (reference 18/WA/0214) and the NHSBT ethics committee (reference 06/Q702/61)

coli DNA polymerase I (52)

placebo at week 72 (95.3% vs