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Off-Label Prescribing: Many longevity treatments including metformin, rapamycin, low-dose naltrexone, and daily tadalafil are manufactured in FDA-registered facilities for specific approved conditions but are prescribed off-label within longevity-focused or age-related wellness discussions

To produce recombinant CspZ proteins without affinity tags for VLP conjugation and vaccination, an cystein has been added to C-termini of both CspZ and CspZ-Y207A/Y211A for coupling these proteins to VLPs as described (25, 26)

e , Representative bright field images of glucose-deprived vector- or AMPK-expressing H1299 cells pretreated with indicated -KG competitors

A major focus for future clinical translation will be to enhance the design and application of targeted therapies while minimizing the toxic side effects associated with copper-targeting agents