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To this end, we analyzed WT and KO cells at day 8 of differentiation, 24 h after directed pancreatic fate induction by a combination of Retinoic Acid (RA) and Protein Kinase C (PKC) pathways activation along with BMP and Sonic hedgehog (SHH) pathway inhibition, using single-cell combinatorial indexing RNA sequencing (scRNA-seq) (Fig