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The Fenton reaction, triggered by haem degradation and ferritinophagy mediated by nuclear receptor coactivator 4 (NCOA4), can result in heightened vulnerability to ferroptosis (301308)

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After overnight incubation, cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilised with 0.2% TritonX-100, blocked with 5% of goat serum and incubated overnight at 4 C with the anti-mature IGF-1 antibody (Prepotech)