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and residues Asp461 and Asp433, the latter being involved in a hydrogen bonding network with the side chains of Lys508 and His547, likely critical for GT2 domain folding stability

FLIM can record fluorescence lifetimes using the time domain technique, time-correlated single-photon counting (TCSPC) (Figure 2 ( Figure 2 Fluorescent substances are excited with a short pulse laser, and the time interval it takes for molecules to excite and emit a photon is captured by a detector, allowing the calculation of the fluorescence lifetime

Some people are fully corrected in a single appointment, while others benefit from a short series spaced over a few weeks

Even though it is curable at an early stage, novel drug identification, clinical success, and drug resistance is another major challenge