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Metabolomics of gWAT revealed that NDI1 expression led to an increase in abundance of several nucleotides (NAD + , ATP, GTP) and succinic acid, a reduction in pyruvic acid and -ketoglutarate, and an increase in the NAD + -to-NADH and ATP-to-ADP ratios (Fig

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Key substrates include transcription factors such as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B), tumor protein 53 (p53), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1), and nuclear factor erythroid 2-related factor 2 (Nrf2), which play integral roles in maintaining cellular homeostasis (Mohammed et al., 2021)