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We propose that Tyr343 may also be involved in the NADH-induced homodimerization and cFSP1 activity regulation, supporting by that Y343A and Y343F mutations reduced its NADH oxidation activity (Supplementary Table 2)

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The highest NADPH concentration used in this reaction was 3.5-fold higher than the enzyme concentration and the flavin reduction phase does not appear to approach a limit in terms of rate or amplitude