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The addition of biological levels of GSH restored pyocyanin to wild-type levels, indicating that PmtA alters the redox environment and influences pyocyanin production (Figure 3B

However, the underlying molecular mechanisms by which autophagic activity regulates these alterations to promote epileptogenesis remain unclear

ZL: Writing original draft, Conceptualization

Slides were treated with HIER buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120)