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Expression analysis of GPX4 mRNA in DLBCL To determine whether GPX4 is overexpressed at the protein level or at the mRNA level in DLBCL, we first evaluated the GPX4 protein expression level in 48 DLBCL cases by immunohistochemistry and, depending on the results, divided into two groups, GPX4-positive and GPX4-negative group, according to the criteria described above

In cases of Vitamin B12 deficiency, higher daily doses of 50 to 250 mcg are required

All procedures involving human subjects conformed to standards for the use of human participants in research, with the exception of pre-registration on a clinical trial registry, as outlined in the Declaration of Helsinki

Some protocols recommend intramuscular injection into the worked muscle ("site injection"), with the theory that local IGF-1R stimulation drives localized hypertrophy