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The fundamental principle of the pull-down assay service involves immobilizing a tagged fusion protein onto an affinity resin to serve as the "bait protein." When the tagged fusion protein is incubated with the target protein, tissue, or cell lysate, the proteins that specifically interact with the bait protein are captured

Bsm BI recognition sites were appended to each sgRNA sequence along with the appropriate overhang sequences for cloning into the sgRNA expression plasmids, as well as primer sites to allow differential amplification of subsets from the same synthesis pool

An excess amount of reactive oxygen species (ROS) in the bloodstream causes DNA damage and oxidative stress in cells (61)