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Subsequently, about 12.5 g of the seedlings were transferred to a single 50-mL reaction tube containing 30 mL di-potassium hydrogen phosphate buffer (0.1 M, pH 9.5, adjusted with 10 M KOH) buffer and 100 mg of glass beads (250500 m diameter)

These gaps highlight the need for future research to move beyond descriptive associations toward systematic dissection of PKD mutationferroptosis crosstalk, cell type-specific functions, and clinical validation