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Because of the time constraints for the MR scanner and the time needed to perform serial MRI scans, not all pups in a litter could undergo behavioral testing or be scanned at all time points

Interestingly, mechanotransduction during myelin formation appears to be regulated opposingly to oligodendrocyte differentiation: pliable substrates promote myelin membrane wrapping of synthetic axons while wrapping is hindered by stiff substrates 30

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Instead, angle the needle so that the tip is resting against the inside glass wall of the vial