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HEK-293 cells were stably transfected with GFP-hBOK using a Flp-In-T-Rex-293 cell line (ThermoFisher Scientific) as previously described 26

Disclosure of interest: Carmen Garca-Cabo, Lorena Benavente Fernndez, Mara Castan Apilanez, Montserrat Gonzlez Delgado, Davinia Larrosa Campo, Elena Lpez-Cancio, Mara Rico Santos, Sergio Calleja Puerta.: nothing to disclose E-Posters - DIAGNOSIS / ETIOLOGY 04.00 - DIAGNOSIS / ETIOLOGY - 04.01 - DIAGNOSIS/INVESTIGATION OF STROKE ETIOLOGY P2383 - ESOC25-1567 REDUCING ACUTE ADVANCED IMAGING BURDEN IN ACUTE NEUROLOGICAL PRESENTATIONS: THE ESSENTIAL ROLE OF TELESTROKE NEUROLOGISTS Shayandokht Taleb 1,2 , Navdeep Sangha 1 , Zahra Ajani 1 , Denise Gaffney 1 , Howard Rho 1 , Jeffrey Saver 2 1 Kaiser Permanente Los Angeles Medical Center, Los Angeles, United States, 2 University of California, Los Angeles, Los Angeles, United States Background and Aims: Stroke mimics account for 5-60% of stroke codes

Iron assay The relative Fe2 + concentrations in tissues and cells were evaluated using the iron assay kit (Ab83366, Abcam)

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