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For transduction, 200 l lentiviral supernatant was thawed on ice and added to HCC-1806 cells together with 8 mg ml 1 Polybrene (hexadimethrine bromide

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Nagy 3 , Svetlana Radaeva 6 and Gyongyi Szabo 1 , (1)Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, (2)University of Massachusetts Memorial Health Care, (3)Cleveland Clinic Foundation, (4)University of Louisville, Louisville, KY, (5)University of Texas Southwestern Medical Center, (6)National Institutes of Health, Bethesda, MD Background: Extracellular vesicle (EVs) cargo consists of RNA and protein molecules from the cells of origin reflecting the pathophysiological state specific to a disease and thereby, are emerging as potential biomarkers