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Quantitative real-time PCR analysis Total RNA was isolated from hippocampal tissue (n=6 per group) and reverse transcribed into cDNA using FastKing gDNA Dispelling RT SuperMix (KR118, Tiangen, China)

The frequency varies depending on your health and goals

10.3390/antiox10040577 42 FanL.SunG.MaT.ZhongF.WeiW

06-Methylguanine-DNA Methyltransferase (MGMT) Gene in Head and Neck Squamous Cell Carcinoma (HNSCC): Promoter methylation and Loss of Protein Expression with Clinical Correlation in 94 Patients