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Assessment of risk of bias One study used a random number table to randomize the animals into groups, and sixty studies did not report their randomization methods and were thus marked "unclear." Fifty-one included studies reported random animal placement, and the remaining studies did not mention such placement

The copper coordination chemistry of GHK-Cu enables controlled cellular copper delivery while preventing the formation of reactive oxygen species characteristic of free copper toxicity, with the peptide's binding affinity (log K = 16.1) positioning it perfectly within the physiological copper transport hierarchy to facilitate controlled metal transfer to enzymatic targets including lysyl oxidase (essential for collagen cross-linking) and superoxide dismutase (critical for antioxidant defense)

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