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Glutathione is one of the powerful antioxidants found in plants and animals

Zhang S, Liu H, Amarsingh GV, Cheung CCH, Wu D, Narayanan U, et al

Ascorbic acid and N-acetylcysteine improve in vitro the function of lymphocytes from mice with endotoxin-induced oxidative stress

And then, 0.5 L phi29 DNA polymerase, 5 L 10 phi29 DNA polymerase buffer (330 mM Tris-acetate, pH 7.9 at 37 C, 100 mM Mg-acetate, 660 mM K-acetate , 1% (v/v) Tween 20, 10 mM DTT), 2.5 L dNTPs (with a final concentration of 500 M) were introduced to the above mixture (total volume: 50 L)