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In sum, long-term infertility is associated with a modified methylome in euploid blastocysts with specific concentration on regulation pertaining to genomic imprinting, which is compared to aided reproductive technologies alone (Reference Denomme, Haywood and McCallie37)

To confirm that tree-based ML models are the most appropriate for our analysis we also built Least Absolute Shrinkage and Selection Operator (LASSO) models to predict circulating TMAO in the left-out group using again fivefold cross-validation with all the available variables (full model) as input in BMIS ( N = 582

sub-phenotype-3 (23.4%) with skeleto-muscular and nervous disorders