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The rats were maintained in individual cages, under controlled temperature, humidity and illumination conditions, with water and diet ad libitum for eight weeks

doi: 10.1016/j.vaccine.2008.11.008 118 Leroux-RoelsG.Van DammeP.HaazenW.ShakibS.CaubetM.ArisE.et al

First, 1057 Ecad cells were either pretreated with ICI for three days or left untreated before being injected into the tail vein of female C57BL/6J mice, simulating adjuvant therapy after primary tumour surgery