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SKU: 21061309058 · From www.asociacionchamorro.org

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Description

For CRISPR/Cas9 mediated knock out of both C/EBP isoforms, guide RNA squences (LAP: 5-GAGTGGCCAACTTCTACTACG-3, LIP: 5-GCGCTTACCTCGGCTACCAGG-3) were cloned into pSpCas9(BB)-2A-puro (PX459) v2.0 ( Transfection Immortalized MEFs were transfected with an empty, rat C/EBP-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol