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Serious illness and death have occurred as a direct result of some portions of the treatment, including severe electrolyte imbalances

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After centrifuged at 125,000 x g for 1 hour, the supernatant was removed, and pellets were diluted to a final lipid concentration of 2.5 mg/ml in wash buffer consisting of 150mM KCl and 50mM HEPES (pH 7.4)

Thus, the low expression of CD320 observed in EAE mice and MS patients could potentially cause impaired VitB12 uptake within the CNS, although, according to Scalabrino and co-workers, no change in VitB12 content was detected in the blood and cerebrospinal fluid (CSF) of MS patients [95]