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The secondary antibodies included goat anti-rabbit Alexa Fluor 488 (Abcam, Ab150077, 1:500), goat anti-rabbit Alexa Fluor 594 (Abcam, Ab150088, 1:500), and goat anti-mouse Alexa Fluor 594 (Abcam, Ab150116, 1:500)

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Month 1 (First PRP Session at Week 45) Healing phase finishing Shock loss starting PRP #1: growth factors begin working over several days Benefit: better micro-circulation, reduced inflammatory drag Month 2 (PRP Session #2) Shock loss typically at its worst PRP #2: supports follicles as they shift through telogen Benefit: may lessen depth and duration of shedding Month 34 (PRP Session #3) Early new hairs sprouting PRP #3: supports anagen onset Benefit: earlier and more robust early growth vs non-PRP cases Month 56 Hair getting thicker and denser If extended protocol: more PRP sessions here Benefit: further support for follicle quality and shaft thickness Month 912 Final density approaching Optional finishing PRP session Benefit: hair quality and shine support in the maturation phase Who Benefits the Most From PRP After Hair Transplant