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This fingerprint should link molecular signatures (e.g., co-expression of ACSL4 and 4-HNE) to histological lesions (e.g., tubular necrosis and fibrosis), enabling precise localization and quantification of ferroptosis in the renal microenvironment

Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded, while anti-MBP (Rat, BioRad MCA4095,1:1000) was used to detect MBP