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cholesterol levels in the NNMT inhibitor-treated DIO mice were similar to cholesterol levels reported by the vendor for age-matched normal chow-fed C57Bl/6 mice (www.jax.org/jax-mice-and-services/find-and-order-jax-mice/most-popular-jax-mice-strains/dio-b6)

Foods like meat, dairy, eggs, and fortified cereals are excellent sources

Rare but serious risks include pancreatitis, gallbladder disease, acute kidney injury (secondary to dehydration from GI effects), and a theoretical thyroid C-cell tumor risk based on rodent data that has not been confirmed in human post-marketing surveillance

After several rinses with 0.01 M PBS, the sections were placed in secondary antibody (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 2 h