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In mitapivat-treated 4.2 / mice, we noted decreased protein oxidation in liver as determined by Oxyblot (Figure 5C and Supplemental Figure 8B), which was accompanied by blunted activation of Nrf2 and NF-B p65 (Figure 5D and Supplemental Figure 8C) with resultant downregulation of Nrf2-related cytoprotectors such as heme-oxygenase-1 (HO-1) and Gpx1 (Figure 5E and Supplemental Figure 8D) (17, 46, 47)

doi: 10.3390/ijms232315445, 164

The C677T variant is a change in the instruction manual that makes the MTHFR protein less able to deal with heat

Your doctor might want you to get your vitamin B12 level tested and adjust the dosage accordingly