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Artefacts with ascorbate and other redox-active compounds in cell culture: epigenetic modifications, and cell killing due to hydrogen peroxide generation in cell culture media

For instance, TNF, primarily generated by activated macrophages, has the potential to increase ROS production in lung epithelial cells, which is a plausible mechanism for inflammation-induced carcinogenesis [41]

Research has examined NNMT inhibition and its downstream effects on cellular NAD+ salvage, methylation balance and adipocyte energy metabolism, with preclinical work exploring roles in lipid handling and metabolic regulation

For each treatment, one column contained 30g/ml of digitonin as a positive control for cytotoxicity