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After the reaction, the acetonitrile was removed by rotary evaporation, an appropriate amount of ethyl acetate was added to dissolve the product, and 4% NaHCO was used for each

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All of the steps described above proceed in chloroplasts [13], but the process of thiamine conversion to TDP occurs in the cytosol and is catalyzed by thiamine pyrophosphokinase, encoded by the TPK genes [14]