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The samples were diluted in RIPA, mixed with Laemmli buffer (Bio-Rad, Hercules, CA), and then denatured at 95C for 5 min

Overall, this trial was designed to precisely determine the dose-response relationship, efficacy, and safety of Cagrilintide compared with placebo and liraglutide, while employing advanced statistical methods to ensure reliable interpretation of treatment effects under both ideal and real-world adherence conditions