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Normal RBCs were treated with 0.01% and 0.02% GA at 25 C for 30 min to increase rigidity, a common method in RBC deformability studies

The supernatant, clarified by centrifugation of the solubilized sample at 48,000 g at 4 C for 1 h, was incubated with Ni-NTA beads for batch binding at 4 C for 1 h with mild stirring

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