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Primer sequences for cloning are listed at Appendix Table S5

La combinacin de cido alfa lipoico con vitamina E es particularmente valiosa para proteccin de membranas mitocondriales que contienen fosfolpidos ricos en cidos grasos poliinsaturados como cardiolipina y que son sitios de generacin intensa de especies reactivas de oxgeno

In addition, SIRT1 reduces oxidative stress and inflammation by inhibiting the nuclear factor kappa B (NF-B) signaling pathway, resulting in decreased production of inflammatory cytokines, including IL-1, interleukin-6 (IL-6), tumor necrosis factor (TNF-), and ROS (133135)

Hatchability was calculated by considering the ratio of chickens hatched to the live chicken's embryo after the treatment and expressed as a percentage of fertilized eggs