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Systemic metabolic alterations and their relationship to neuropsychiatric symptomatology PAIS, including long COVID, as well as CFS and IMD, share the characteristic of systemic metabolic disturbances, which may be a sign of underlying multi-organ inflammation and exhaustion [31], and consequently may contribute to neuropsychiatric symptomatology, as many metabolites can cross the bloodbrain barrier (BBB) [30, 58] (e.g., short chain fatty acids (SCFAs) derived from the gut microbiota such as acetate [40], amino acids such as tryptophan [52] and also carnitine/acetylcarnitine can cross the BBB via transporters [59])

[CrossRef] [PubMed] [Google Scholar] l-Acetylcarnitine: A mechanistically distinctive and potentially rapid-acting antidepressant drug

Then 80% of the supernatant was transferred to a new tube and 2 l of concentrated ammonia solution added, followed by 100 l of 4 mg/ml ethyl bromoacetate (Oakwood chemicals) in ACN and samples vortex mixed

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