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Antigen-specific T cell sorting, sequencing and cloning Expanded T cells were stained with CD3, CD8, CD14, CD19, live/dead, and matched and mismatched dextramer, and single-antigen-specific T cells were sorted using a FACSAria Fusion (BD Biosciences)

Martinez DST, Ellis L-JA, Da Silva GH, Petry R, Medeiros AMZ, Davoudi HH, Papadiamantis AG, Fazzio A, Afantitis A, Melagraki G, Lynch I

In Southeast Asia and sub-Saharan Africa, zinc deficiency is endemic, affecting up to 33% of the population

Formation of hydrogen peroxide was determined after addition to the solution of 10 l of 2-7dichlorofluorescein diacetate (2m in ethanol) and DCF fluorescence measured as described previously (Garca-Ruiz et al, 1995, 1997)