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Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking

Niacinamide is a precursor to NAD+, a critical molecule for cellular repair and the regulation of inflammatory cytokines

also showed significant increases in serum concentrations of asymmetric dimethylarginine (ADMA), enhanced inhibition of NOS, enhanced catabolism of Arg, increased levels of ornithine (Orn) and proline (Pro), and decreased Arg/Orn ratio in patients with asthma and obstructive sleep apnea (OSA)

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