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At the end of experiments, the rats were anaesthetised by isoflurane and euthanised by exsanguination

For example, the amplification of Myc causes cellular addiction to glutamine, which may be related to the combined effects of Myc and glutamine transporter (such as SLC7A5 and SLC1A5) promoter elements, leading to enhanced glutamine uptake, which induces the activation of Lactate Dehydrogenase A (LDHA) and transports glucose-derived citrate out of the mitochondria, thereby increasing the requirement of glutamine [29,30,31]

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In UK clinical practice, sermorelin has been used primarily as a diagnostic agent to assess pituitary GH reserve it is not licensed in the UK for the treatment of growth hormone deficiency in adults or children