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They were cloned into pKO3, and following the published protocol (Link et al ., 1997), this vector was used to sequentially select and then screen for replacement of the wildtype trxC allele with the deletion allele

M.KosinskiJ

Analysis of loss-of-function mutants of these two transporters revealed that the accumulation of GSL was reduced by 50% in the seeds and was significantly increased in source leaves