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The chitin content was determined by measuring the absorbance at 530 nm using a spectrophotometer

Safety and Risks while vitamin injections are generally considered safe, especially when administered by trained medical professionals, it's important to be aware of potential, though rare, side effects

doi: 10.1038/ncomms5430 117 FongLKYangMMDos Santos ChavesRReynaSMLangnessVFWoodruffGet al

The most frequent symptoms are: Developmental delays Anaemia Loss of appetite Chronic vomiting Weight loss Low brain volume Spasms/cramps Apathy Irritability In the most severe instance, vitamin B12 deficiency can lead to respiratory failures and coma (51)