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Str00901 748

Expression of wild-type CDO, but not catalytically inactive His86Ala CDO, reduced intra-cellular Cys levels in HepG2/C3A cells, which do not express endogenous CDO protein, incubated in physiologically relevant concentrations of Cys (Dominy et al

In general, hydrophilic amino acids contribute to the formation of a hydrophobic pocket in the H-site adjacent to the GSH-binding site (Figure 3B&C) [80, 85]

Monitoring the SELEX procedure: melting curve and NGS analyses The melting temperature (Tm) profiles of amplified dsDNA (from eluted ssDNA) from different SELEX rounds were evaluated to decipher the diversity evolution of aptamer pools, as melting curve analysis is a rapid, simple, informative, and efficient approach for assessing the convergence of oligonucleotide pool diversity