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Finally, blastocysts from each group were collected and incubated with 5 g/ml Hoechst 33342 solution at 38C for 5 min, followed by blastocysts mounting and microscope examination with cell number per blastocyst ( n = 20 for each group) further recorded

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Acknowledgments We gratefully acknowledge Laboratori Farmaceutici Krymi (Rome, Italy) for their technical assistance and for kindly providing GSH-C4 and all clinical study medical devices as a gift