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In: HusenA, editor

demonstrated that Tom20 or Bax knockout effectively blocks ROS-induced pyroptosis, while antioxidant treatment significantly reduces Tom20 oxidation, Bax mitochondrial recruitment, and GSDME cleavage, further confirming that ROS serves as the central regulator of this signaling pathway (47)

We can observe that over the years, mitochondrial assessment has evolved, and new methodologies and techniques have been improved, as in the work of Mendelsohn et al., who investigated the role of energy detection in the response of the zebrafish embryo to anoxia, testing the arrest of development in mitochondrial inhibitors, through oxygen consumption measured by respirometer equipment [114]

Other than this, we observed no alterations in serum glucose, total protein, albumin, globulin, or albumin: globulin ratio across groups, suggesting that Se level in CON_HS was adequate for maintaining these attributes during HS, as also reported by Kumar et al