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The supernatant was carefully removed, and the pellet was resuspended in 300 L of 2% SDS buffer in PBS for immunoprecipitation assays, or in 300 L of 8 M UREA in PBS with 10 mM TrisHCl pH 7.4

For the former the study design may be focused on obtaining sufficient samples in order to be representative of the mycotoxin of interest

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Per-session flow (non-instructional):cleanse antisepsis apply reconstituted vial in thin layers according to chosen method soothe SPF on exposed areas