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The second-generation anti-androgen drugs such as enzalutamide or abiraterone in standard treatment of advanced prostate cancer combined with ferroptosis activator can further inhibit tumor proliferation [17]

We treated A2780 ARID1A-negative cancer cells with either vehicle or EN25, and subsequently treated resulting cell lysates with the alkyne functionalized cysteine-reactive iodoacetamide probe, after which isotopically encoded cleavable biotin-azide enrichment handles were appended onto probe-labeled proteins by click-chemistry, and subjected to avidin-enrichment, tryptic digestion, elution of probe-modified tryptic peptides, and analysis by quantitative proteomic methods

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