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Always use low-binding microcentrifuge tubes and pipette tips (siliconized or polypropylene) when handling dilute solutions below 10 mcg/mL, as surface adsorption can reduce effective concentrations by 20-50% in standard labware

Acta Pharmacol Sin 44:22302242 Kolbrink B, von Samson-Himmelstjerna FA, Messtorff ML, Riebeling T, Nische R, Schmitz J, Brsen JH, Kunzendorf U, Krautwald S (2022) Vitamin K1 inhibits ferroptosis and counteracts a detrimental effect of phenprocoumon in experimental acute kidney injury