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The preparation process of freeze-drying includes three steps: dissolving polymers in water to acquire a polymer solution, freezing the solution in a container or a mold, and removing the ice from the frozen solution with a lyophilizer

In an in vivo study comparing the blood glucose levels-lowering effects of saline, PO-free insulin solution, SC insulin solution, uncoated NCs, and pHPMA nanoparticles with the lowest charge density in diabetic rats, pHPMA nanoparticles demonstrated a maximal reduction of 50% in blood glucose levels, at 4 h post-administration with a dosage of 75 IU/kg

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This subsequently contributes to the pathophysiological progression of type 2 diabetes, obesity-related metabolic syndrome, and microvascular complications (diabetic nephropathy, retinopathy)