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Du Vigneaud was awarded the Nobel Prize in Chemistry for his work on the synthesis of essential biological substances, including his research on methionine and homocysteine [5]

5FU has previously been demonstrated to have a significant metabolic impact shortly after treatment, rapidly and consistently inhibiting TS 5

To investigate the potential ligating Cys in GSDME, AlphaFold structure of GSDME (AF-O60443-F1-model_v2) was used as the initial structure in view that the experimental GSDME structure remains still unresolved