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Maintenance and Expression of Mammalian Mitochondrial DNA

After washing once with RIPA buffer, three times with 1% (v/v) SDS, 8 M Urea in PBS, once with 1% (v/v) SDS in PBS and two times with PBS, pulled down proteins were either eluted by 2x Laemmli, including 100 M biotin, for western blot analysis or on-bead digested for mass spectrometry evaluation (see mass spectrometry methods)

Treated female mice that were given DSIP from three months of age until they died, for 5 consecutive days each month, showed a 2.6-fold decrease in tumor development and a 22.6% lower frequency of chromosomal defects in the bone marrow cells

Neural stem cell-derived EVs, on the other hand, deliver functional mitochondria to recipient cells, thereby modulating metabolic activity [51]